Integrated Multi-Assay Culture Model for Stem Cell Chondrogenic Differentiation
نویسندگان
چکیده
منابع مشابه
Hybrid Protein–Glycosaminoglycan Hydrogels Promote Chondrogenic Stem Cell Differentiation
Gelatin-hyaluronic acid (Gel-HA) hybrid hydrogels have been proposed as matrices for tissue engineering because of their ability to mimic the architecture of the extracellular matrix. Our aim was to explore whether tyramine conjugates of Gel and HA, producing injectable hydrogels, are able to induce a particular phenotype of encapsulated human mesenchymal stem cells without the need for growth ...
متن کاملModelling Mesenchymal Stem Cell Expansion and Chondrogenic Differentiation
Introduction In vitro expansion and differentiation of mesenchymal stem cells (MSCs) rely on specific environmental conditions. One crucial factor is the oxygen tension. Low oxygen tension improves MSC expansion in vitro. Moreover, it accelerates the induction of a chondrogenic phenotype in subsequent 3D differentiation cultures. However, clones of MSCs from the same donor often differ in their...
متن کاملThe Effect of Platelet Rich Plasma on Chondrogenic Differentiation of Human Adipose Derived Stem Cells in Transwell Culture
Objective(s): Platelet-rich plasma (PRP) has recently emerged as a promising strategy in regenerative medicine due to its multiple endogenous growth factors. Little is known about the role of PRP as a promoter in chondrogenesis of human adipose derived stem cells (hADSCs). The aim of this study was to determine whether PRP may be considered as a natural and easy achievable source o...
متن کاملInduction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-β3 in Pellet Culture System
Objective Adult stem cells which are derived from different tissues, with their unique abilities to self-renew and differentiate into various phenotypes have the potential for cell therapy and tissue engineering. Human adipose tissue is an appropriate source of mesenchymal stem cells with wide differentiation potential for tissue engineering research. In this study isolated stem cells from hum...
متن کاملCulture and Assay Systems Utilized for Cancer Stem Cell Research
Advances in stem cell biology over the last two decades have provided new insights into cancer biology. Tumors show marked heterogeneity in morphology, proliferation rates, genetic lesions and therapeutic response. Thus, not all tumor cells are equal. This heterogeneity is even seen in an individual tumor that is clonal. The cellular and molecular basis for tumor heterogeneity represents a fund...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2019
ISSN: 1422-0067
DOI: 10.3390/ijms20040951